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KMID : 0602720040080010058
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Implantology
2004 Volume.8 No. 1 p.58 ~ p.71
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Experimental study of PDGF’s effect on healing potential of the allogeneic demineralized Freeze-dried bone powderstransplanted in the calvarial defect of the rat
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Hyun Jae-Hoon
Yim Chang-Joon
Lee Jong-Jin
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Abstract
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PDGF is a mitogen for connective tissue cells, which was produced by macrophage and stored in platelet, and acts as wound hormone increasing the number of mesenchymal cells during initial healing period. It is released by platelet to would area and functions as chemoattractant for fibroblasts, polymorphoneutrophils, monocytes. It also enhances DNA synthesis and collagen synthesis and acts as an important factor associated with migration of osteoblast during bone remodeling. This study was designed to find out the effect of PDGF on bone healing potential of the demineralized bone powders which were transplanted into calvarial defect of the rats. We filled 5mg of DFDB into the calvarial defect with 4mm diameter in 9 rats(exp.Ⅰgroup), DFDB 5mg plus recombinant human PDGF-BB 100ng 20㎕ in anther 9 rats (exp.Ⅱ group) and made defect without filling any graft materials(control group). We sacrificed them and removed the previously- operated area involving the whole defect in 3 rats at the date of postoperative 7 days, 14 days, 21 days. The removed specimens were stained with H&E, on Gomori’s trichrome(GT) and the amount of newly formed bone in the defect area was evaluated under the light microscope and histomorphometric analysis was carried out. So, we got the results followed by 1) In every group of calvarial defect, the amount of new bone formation was increased as the time being. 2) In exp.Ⅱ and control group at 21 days after operation, bridging images connecting both cutting edges of calvarial defects were observed. 3) The largest amount of newly formed bone was observed in exp.Ⅱ group, less in control group and the least in exp.Ⅰgroup. 4) In the histomorphometric analysis, there is significant difference between control and exp.Ⅱ group, exp.Ⅰand exp.Ⅱ group in 1 week group and 2 weeks group in 2 week, but there is no significant difference among the groups. From the above results, it is suggested that PDGF promote new bone formation of allogenic demineralized freeze-derived bone powders transplanted in bone defect area and demineralized bone act as carrier of PDGF
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KEYWORD
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PDGF, DFDB, new bone formation, carrier
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